Biochem/physiol Actions

Primary Targetprotein biosynthesis that acts on the 30S ribosome

General description

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity.

Contains >98% kanamycin A. Potency: ≥750 µg/mg

Recommended working concentration: 100 µg/ml.

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 70S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity. Useful for the selection of kanamycin resistant plasmids in E. coli. Tested and found to be negative for cytotoxicity against HeLa cells. Contains more than 98% kanamycin A.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.Cardon, G.H., et al. 1993. Plant J.3, 773.Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties1, 690.Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother.6, 516.Umezawa, S., et al. 1968. J. Antibiot.21, 367.

Packaging

5, 25 g in Glass bottle

Reconstitution

Following reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Warning

Toxicity: Carcinogenic / Teratogenic (D)